Development of Taqman RT-nested PCR system for clinical SARS-CoV detection
نویسندگان
چکیده
منابع مشابه
Development and comparison of the real-time amplification based methods--NASBA-Beacon, RT-PCR taqman and RT-PCR hybridization probe assays--for the qualitative detection of sars coronavirus.
The aim of this study was to develop a rapid, sensitive and robust procedure for the qualitative detection of SARS coronavirus RNA. Three unique detection formats were developed for real-time RNA amplification assays: a post amplification detection step with a virus-specific internal capture probe based on Taqman (RT-PCR TaqMan assay), hybridization probe (RT-PCR hybridization probe assay) and ...
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ژورنال
عنوان ژورنال: Journal of Virological Methods
سال: 2004
ISSN: 0166-0934
DOI: 10.1016/j.jviromet.2004.02.011